Data di Pubblicazione:
2012
Citazione:
Genetic investigation within Lactococcus garvieae revealed two genomic lineages / C. Ferrario, G. Ricci, F. Borgo, A. Rollando, M.G. Fortina. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - 332:2(2012), pp. 153-161.
Abstract:
The diversity of a collection of 49 Lactococcus garvieae strains, including isolates
of dairy, fish, meat, vegetable and cereal origin, was explored using a molecular
polyphasic approach comprising PCR-ribotyping, REP and RAPD-PCR analyses
and a multilocus restriction typing (MLRT) carried out on six partial genes
(atpA, tuf, dltA, als, gapC, and galP). This approach allowed high-resolution
cluster analysis in which two major groups were distinguishable: one group
included dairy isolates, the other group meat isolates. Unexpectedly, of the 12
strains coming from fish, four grouped with dairy isolates, whereas the others
with meat isolates. Likewise, strains isolated from vegetables allocated between
the two main groups. These findings revealed high variability within the species
at both gene and genome levels. The observed genetic heterogeneity among
L. garvieae strains was not entirely coherent with the ecological niche of origin
of the strains, but rather supports the idea of an early separation of L. garvieae
population into two independent genomic lineages.
Introduction
In the last two decades, foodborne diseases have been
emerging as an important and growing public health concern.
Among the causes, changes in agricultural and zootechnical
practices, increase in international trade,
changes in consumer lifestyles, and increasing number of
old and/or immune-compromised consumers have been
mentioned. Furthermore, new pathogens or new biovars
of known bacterial species are frequently being reported
(Mor-Mur & Yuste, 2010). The impact of most new
pathogens on specific ecosystems and their pathogenicity
are not known. Indeed, the traditional food inspection
systems are insufficient, because knowledge of the emerging
pathogens is incomplete. Furthermore, the new techniques
based on DNA analysis are not always applicable,
in the absence of genetic data on these new biotypes.
Therefore, to determine the concept of healthy food, it is
crucially important that we expend efforts to comprehensive
study of new emerging pathogens present in food
products.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Emerging food pathogen; Genomic lineages; Lactococcus garvieae; MLRT; Molecular fingerprinting
Elenco autori:
C. Ferrario, G. Ricci, F. Borgo, A. Rollando, M.G. Fortina
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