Evaluation of bovine-derived lacteal complex supplementation on gene expression in BALB/c mice.
Articolo
Data di Pubblicazione:
2011
Citazione:
Evaluation of bovine-derived lacteal complex supplementation on gene expression in BALB/c mice / M. Clerici, E. Pauze, A. de Jong, M. Biasin, L.E. Miller. - In: NUTRITION AND DIETARY SUPPLEMENTS. - ISSN 1179-1489. - 2011:3(2011), pp. 89-92. [10.2147/NDS.S23701]
Abstract:
We conducted an evaluation of gene expression associated with innate and adaptive immunity in a double-blind ex vivo mouse study using a bovine-derived dietary ingredient (Ai/E10®, Health Technology Resources, Inc., Scottsdale, AZ, USA). BALB/c female mice (5–6 weeks of age) were fed chewy granola bars supplemented with (Test) or without (Control) Ai/E10 for 10 days. After the feeding period, the animals were sacrificed and spleen cells were isolated and incubated with lipopolysaccharide and phorbol myristate acetate-ionomycin. RNA was extracted and mRNA expression of 84 genes involved in innate and acquired immunity was determined with real-time PCR arrays. Numerous genes associated with innate and adaptive immunity were upregulated in the Test group when stimulated with mitogens. Significant upregulation was observed in 30% (25 of 84) of genes upon lipopolysaccharide stimulation and in 14% (12 of 84) of genes upon phorbol myristate acetate + ionomycin stimulation in the Test group relative to Controls. This study illustrates that Ai/E10 supplementation results in significant and specific upregulation of genes associated with innate and adaptive immunity in mice. Notably, this effect was observed only in stimulated cultures.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
dietary supplementation ; immunomodulation ; mice
Elenco autori:
M. Clerici, E. Pauze, A. de Jong, M. Biasin, L.E. Miller
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