Factors Affecting the Proliferation and Rooting of Chestnut in Vitro

In order to improve shoot proliferation of chestnut and to investigate the influence of nitrogen on the appearance of vitrified shoots, different N and NH4+ levels on MS modified media were compared. The highest proliferation rates and the lowest vitrification incidence were observed on media containing low total N levels and low NH4+/NO3- ratios. Shoots from embryos showed better proliferating and rooting ability compared with shoots from axillary buds of three different clones.