Culture strategies for maturation of carnivore oocytes

In vitro maturation (IVM) of carnivore oocytes is still under investigation. It is well known that oocytes must accomplish nuclear and cytoplasmic maturation to acquire developmental competence. However, little is known about mechanisms regulating these events in carnivore oocytes. Consequently, IVM rates are still lower than those obtained in other species. To improve results in carnivores, two strategies have to be investigated: one finalized towards preserving in vitro functional integrity and potentiality to accomplish complete maturation of cumulus–oocyte complexes (COCs), the other finalized towards providing culture conditions adequate for sustaining complete maturation of these oocytes. Thus, modifications of the culture environment during IVM, by addition of substances that stimulate endogenous systems of cell defence and modulate the intracellular levels of regulatory molecules, or by use of sequentially different culture systems, are interesting strategies for enhancing viability and competence in terms of complete maturation of carnivore oocytes. This review is focused on recent advances in the study of these aspects developed in feline and/or canine oocytes.