Data di Pubblicazione:
1988
Citazione:
Inhibition of DNA polymerization and DNA transcription to RNA by seminal plasma peptides / G. Lugaro, F. Campagnari, R. Moretti, M. M. Casellato. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - 950:3(1988 Sep 07), pp. 420-428.
Abstract:
An oligopeptide fraction purified from the extracellular compartment of bull semen and strongly interacting with DNA was shown to hinder mononucleotide polymerizations to DNA and RNA in vitro. The fraction, collectively called seminal plasma inhibitor, was active in the endogenous DNA and RNA polymerase reactions of the nuclei from rat hepatocytes and in the analogous nucleotide polymerizations catalyzed by purified enzymes of bacterial origin. The type of the induced inhibition was studied using the RNA polymerase from Escherichia coli as a representative nucleotidyl transferase. In the enzymatic polycondensation of mononucleotides, the seminal plasma inhibitor appeared to exert its effect mainly by a competitive inhibition for the utilization of DNA templates without specificity with respect to the source and the base sequence of DNA. Concavities of the plots of V0/Vi versus the amounts of inhibitor in the nucleotide polymerizing reactions and of the Dixon plots in the assays of RNA polymerase from E. coli suggested that the isolated oligopeptide fraction contained more than one active molecular species with differential effects at low and high doses. Preliminary results on the microheterogeneity of the seminal plasma inhibitor supported this contention.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Animals; Amino Acids; Transcription, Genetic; Cell Nucleus; Chromatography, High Pressure Liquid; Rats; Cattle; Kinetics; Liver; Semen; Peptides; DNA Replication; DNA-Directed RNA Polymerases; Male; DNA-Directed DNA Polymerase
Elenco autori:
G. Lugaro, F. Campagnari, R. Moretti, M. M. Casellato
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