Determination of enzyme activity, proteinuria and creatininuria in bovine urine

Blood samples were taken just before slaughter and urine samples were taken after slaughter from 67 healthy Italian Friesian cows aged 4-7 years. None of the cows had any clinical or haematological signs of kidney damage, but 46 had macroscopic or histological signs of kidney damage. The remaining 21 animals were used to calculate serum and urine reference values for urea, creatinine, proteins, N-acetyl-glucosaminidase, gamma-glutamyl transferase, lactate dehydrogenase, alpha-hydroxybutyrate dehydrogenase and alkaline phosphatase.