RECIPE FOR A PH 3-4 IMMOBILIZED GRADIENT FOR ISOELECTRIC-FOCUSING

A linearized, optimized recipe for a pH 3-4 gradient is described. In this formulation, water is added as a basic Immobiline with pK = 0.00 (when the concentration is expressed as the activity coefficient on the molar fraction scale, i.e., 1 M) or as a basic Immobiline with pK = -1.74 (when the concentration is expressed as the molarity of water in pure water, i.e., 55.56 M).