ISOELECTRIC-FOCUSING WITH IMMOBILIZED PH GRADIENTS

The technique of isoelectric focusing (IEF) via immobilized pH gradients (IPG) is reviewed. The review shows that in five years, the technique has been extensively developed in three fundamental aspects: analytical, preparative and as a first dimension of two-dimensional (2-D) maps. The merits and the flaws of the IPG technique have been evaluated and recognized to show that the technique is ready for successful introduction in most life-science laboratories. The review, after explaining the basics, gives the most recent advances in the IPG field.