Plasma membrane-associated glycohydrolases activation by extracellular acidification due to proton exchangers
Articolo
Data di Pubblicazione:
2012
Citazione:
Plasma membrane-associated glycohydrolases activation by extracellular acidification due to proton exchangers / M. Aureli, N. Loberto, R. Bassi, A. Ferraretto, S. Perego, P. Lanteri, V. Chigorno, S. Sonnino, A.E.G. Prinetti. - In: NEUROCHEMICAL RESEARCH. - ISSN 0364-3190. - 37:6(2012), pp. 1296-1307.
Abstract:
In this paper, we show that the pH optimum for the plasma membrane (PM)-associated activity of four glycohydrolases (conduritol B epoxide sensitive β-glucosidase, β-glucosidase GBA2, β-hexosaminidase and β-galactosidase) measured on intact cells is acidic. Moreover, we show that drugs able to modify the efflux of protons across the PM, thus locally affecting the extracellular proton concentration close to the PM, are able to modulate the activities of these enzymes. These data strongly suggest that pH-dependent modulation of PM-associated glycohydrolases activities could be an effective way to locally modulate the cell surface glycoconjugate composition.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Plasma membranes; Glycoconjugates; Glycoproteins; Glycolipids; Glycohydrolases; Optimal pH; Proton modulators
Elenco autori:
M. Aureli, N. Loberto, R. Bassi, A. Ferraretto, S. Perego, P. Lanteri, V. Chigorno, S. Sonnino, A.E.G. Prinetti
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