TYPICAL AND ATYPICAL SH3 INTERACTIONS: EVOLUTION AND SPECIFICITY OF STRUCTURAL AND BIOCHEMICAL PROPERTIES

As part of PENELOPE network we focused on biophysical characterization of SH3 domains involved in actin independent polarization. In particular we were interested to characterize at the structural and biochemical level the interactions between the proteins Bem1, Cdc42 and Cdc24, which are partially mediated by canonical and non-canonical SH3 interactions. The ultimate goal was to compare in the four yeasts the nature of this small protein's interaction network, in order to shed light on the nature and evolution of this network in the four yeast organisms, which very likely evolved from a common ancestor.