High-Resolution Mass Spectrometry Non-Targeted Detection of Per- and Polyfluoroalkyl Substances in Roe Deer (Capreolus capreolus)
Articolo
Data di Pubblicazione:
2024
Citazione:
High-Resolution Mass Spectrometry Non-Targeted Detection of Per- and Polyfluoroalkyl Substances in Roe Deer (Capreolus capreolus) / R. Pavlovic, S. Draghi, A. Pellegrini, C. Fornesi Silva, F. Di Cesare, G. Curone, F. Arioli, M. Fidani. - In: MOLECULES. - ISSN 1420-3049. - 29:3(2024 Jan), pp. 617.1-617.15. [10.3390/molecules29030617]
Abstract:
Among wildlife species, roe deer stands out as a valuable indicator of environmental
pollution due to its ecological significance and role as a game animal. The assessment of poly- and
perfluoro substances (PFASs) bioaccumulation is of the utmost importance, relying on the liver
and muscles as the main organs of interest. The study concerned the identification of 60 PFAS
through a non-target workflow analysis based on HPLC Q-Exactive Orbitrap High-Resolution Mass
Spectrometry in a homogeneous group of 18 female roe deer species. The developed strategy allowed
us to individuate the 60 PFAS compounds with different levels of confirmation. Apart from seven
PFASs identified via analytical standards, the remaining fifty-three features were identified with
CL 2 or 3. Moreover, by applying a differential statistic approach, it was possible to distinguish the
bioaccumulation patterns in the liver and muscle, identifying 12 PFAS upregulated in the muscle
and 20 in the liver. The analysis reveals that specific PFAS compounds present exclusively in either
the muscle or in the liver. The study emphasises the specificity of the liver and muscle as significant
bioaccumulation sites for PFAS, raising questions about the underlying mechanisms of this process.
In conclusion, the presented non-targeted PFAS analysis workflow evidenced promising and reliable
results, successfully demonstrating its feasibility in the field of environmental research.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
high-resolution mass spectrometry; non-targeted analysis; compound discoverer; persistent organic pollutants; endocrine disruptors; biomonitoring; wild animals; ecotoxicology
Elenco autori:
R. Pavlovic, S. Draghi, A. Pellegrini, C. Fornesi Silva, F. Di Cesare, G. Curone, F. Arioli, M. Fidani
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