Data di Pubblicazione:
2008
Citazione:
Diagnosi di PMWS in Italia / G. Sarli, F. Ostanello, F. Morandi, L. Fusaro, B. Bacci, A. Nigrielli, L. Alborali, M. Dottori, F. Vezzoli, G. Barigazzi, L. Fiorentini, V. Sala, G. Leotti. - In: LARGE ANIMALS REVIEW. - ISSN 1124-4593. - 14:3(2008), pp. 119-126.
Abstract:
From the beginning of October 2006 to January 31st 2008, in the context of a work sponsored by Merial Italia, a diagnostic pathway has been developed for the study of post-weaning multisystemic wasting syndrome (PMWS) of pigs. Superficial inguinal and bronchial lymph nodes, ileum, tonsil and lung from 3 to 5 subjects (229 pigs belonging to 64 farms from North and Centre Italy) were collected. The samples were valued by histological and immunohistochemical (IHC) assays for porcine circovirus-2 (PCV2). The diagnosis confirmed PMWS in those cases with inclusion bodies or where the presence of a positive IHC stain was contextual to the lesions indicative of PMWS in the lymphoid tissue (depletion and/or granulomas) and/or lung (interstitial pneumonia). In the other cases the samples were considered PCV2 infected when PCV2-positive cells were present without PMWS lesions; negative in the absence of PCV2 infection and PMWS lesions. PMWS was diagnosed on 48 farms; 21 where the total number of examined subjects had a conclusive diagnosis of PMWS, whereas the diagnosis could be considered conclusive for PMWS only for some animals on the remaining 27. The protocol proved more reliable when appled to a group of animals than to a single pig.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Diagnosis; Histopathology.; Immunohistochemistry.; PCV2.; Pig.; PMWS.
Elenco autori:
G. Sarli, F. Ostanello, F. Morandi, L. Fusaro, B. Bacci, A. Nigrielli, L. Alborali, M. Dottori, F. Vezzoli, G. Barigazzi, L. Fiorentini, V. Sala, G. Leotti
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