IgE response to Aed al 13 and Aed al 14 recombinant allergens from Aedes albopictus saliva in humans
Articolo
Data di Pubblicazione:
2023
Citazione:
IgE response to Aed al 13 and Aed al 14 recombinant allergens from Aedes albopictus saliva in humans / I. Arnoldi, M. Villa, G. Mancini, I. Varotto-Boccazzi, M. Yacoub, C. Asperti, A. Mascheri, S. Casiraghi, S. Epis, C. Bandi, L. Dagna, F. Forneris, P. Gabrieli. - In: THE WORLD ALLERGY ORGANIZATION JOURNAL. - ISSN 1939-4551. - 16:11(2023), pp. 100836.1-100836.12. [10.1016/j.waojou.2023.100836]
Abstract:
Background: Mosquito bite is normally associated with mild allergic responses, but severe
localized or systemic reactions are also possible. Reliable tools for the diagnosis of mosquito al-
lergy are still unavailable. Here, we investigated the IgE response to 3 potential salivary allergens
identified in the saliva of the tiger mosquito Aedes albopictus.
Methods: Serum from 55 adult individuals (28 controls and 27 allergic people), were analysed
using an in-house Enzyme Linked ImmunoSorbent Assay (ELISA) against the Salivary Gland Extract
(SGE) and the recombinant proteins albD7l2 (Aed al 2), albAntigen5-3 (Aed al 13) and albLIPS-2
(Aed al 14).
Results: Fifteen of the 27 (56%) individuals having hypersensitive reactions to mosquito bites had
IgE serum levels recognizing SGE. Negative sera did not show detectable levels of IgE targeting
the SGE from the most common sympatric mosquito Culex pipiens. Among the positive in-
dividuals, 2 subjects displayed IgE targeting Aed al 2 (13%), while IgE recognizing Aed al 13 and
Aed al 14 were detected in ten (67%) and seven (47%) individuals, respectively. Two sera from
non-hypersensitive subjects had detectable levels of IgE targeting Aed al 13, suggesting possible
cross-reaction with the homologue salivary proteins of multiple mosquito species or, more
generally, of hematophagous insects.
Conclusions: Our results indicate that Aed al 13 and Aed al 14 hold the potential to be devel-
oped as tools for the diagnosis of allergy to Ae. albopictus bites. Such tools would facilitate
epidemiological studies on tiger mosquito allergy in humans and might foster the development of
further protein-based assays to investigate cross-species allergies.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Venom hypesentivity; Insect proteins; Saliva; Culicidae; Allergens
Elenco autori:
I. Arnoldi, M. Villa, G. Mancini, I. Varotto-Boccazzi, M. Yacoub, C. Asperti, A. Mascheri, S. Casiraghi, S. Epis, C. Bandi, L. Dagna, F. Forneris, P. Gabrieli
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