Evaluation of ex vivo human skin permeation of genistein and daidzein

The percutaneous absorption of genistein (GEN) and daidzein (DAI), whose oestrogenic-like activity is well known, is scantily investigated. In this work the ability of GEN and DAI to reach therapeutic steady-state plasma concentrations following transdermal administration was studied. The skin permeation studies were conducted by using modified Franz diffusion cell and human epidermis as a membrane. PEG400 was the most effective vehicle for both molecules. On the basis of the ex vivo permeation results and estimating therapeutic plasma concentration, we assume that pure GEN can be efficaciously administered by the transdermal route