Multicenter comparative evaluation of six commercial systems and the national committee for clinical laboratory standards m27-a broth microdilution method for fluconazole susceptibility testing of Candida species
Articolo
Data di Pubblicazione:
2002
Citazione:
Multicenter comparative evaluation of six commercial systems and the national committee for clinical laboratory standards m27-a broth microdilution method for fluconazole susceptibility testing of Candida species / G. Morace, G. Amato, F. Bistoni, G. Fadda, P. Marone, M.T. Montagna, S. Oliveri, L. Polonelli, R. Rigoli, I. Mancuso, S. La Face, L. Masucci, L. Romano, C. Napoli, D. Tato, M.G. Buscema, C.M. Belli, M.M. Piccirillo, S. Conti, S. Covan, F. Fanti, C. Cavanna, F. D'Alo, L. Pitzurra. - In: JOURNAL OF CLINICAL MICROBIOLOGY. - ISSN 0095-1137. - 40:8(2002 Aug), pp. 2953-2958.
Abstract:
Fluconazole susceptibility among 800 clinical Candida isolates (60% C. albicans) and two control strains (C. krusei ATCC 6258 and C. parapsilosis ATCC 22019) was tested with the NCCLS M27-A method (gold standard) and six commercial products (Candifast, disk, Etest, Fungitest, Integral System Yeasts, and Sensititre YeastOne). Results were classified as susceptible, susceptible-dose dependent, or resistant using M27-A breakpoints or, for Fungitest, Integral System Yeasts, and Candifast, as susceptible, intermediate, or resistant, according to the manufacturers' instructions. Concordance with NCCLS M27-A results was analyzed with the chi(2) test. Intra- and interlaboratory reproducibility was also evaluated. NCCLS M27-A (90.1%), Etest (93.1%), Sensititre YeastOne (93.1%), disk (96.7%), Fungitest (92.6%), Integral System Yeasts (40.6%), and Candifast (6.0%) classified the indicated percentages of C. albicans isolates as susceptible. Among non-C. albicans strains, the percentages of susceptible isolates were as follows: NCCLS M27-A, 74.0%; Etest, 83.8%; Sensititre YeastOne, 64.1%; disk, 60.6%; Fungitest, 76.6%; Integral System Yeasts, 28.3%; and Candifast, 27.4%. All methods except Candifast and Integral System Yeasts showed good agreement with NCCLS M27-A results for both C albicans and non-C. albicans isolates. Intralaboratory reproducibility was excellent for NCCLS M27-A, Etest, Sensititre YeastOne, disk, and Fungitest (88 to 91%). Similar results emerged from the interlaboratory reproducibility evaluation. Our findings indicate that some commercial methods can be useful for fluconazole susceptibility testing of clinical Candida isolates. Those characterized by a lack of medium standardization and/or objective interpretative criteria should be avoided. Particular caution is necessary when testing is being done for clinical and epidemiological purposes.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
G. Morace, G. Amato, F. Bistoni, G. Fadda, P. Marone, M.T. Montagna, S. Oliveri, L. Polonelli, R. Rigoli, I. Mancuso, S. La Face, L. Masucci, L. Romano, C. Napoli, D. Tato, M.G. Buscema, C.M. Belli, M.M. Piccirillo, S. Conti, S. Covan, F. Fanti, C. Cavanna, F. D'Alo, L. Pitzurra
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