Shoot Regeneration, in Vitro Performances of Regenerated Shoots and Transient Expression in Morphogenic Explants in Prunus avium cultivar 'Burlat C1'
Articolo
Data di Pubblicazione:
2010
Citazione:
Shoot Regeneration, in Vitro Performances of Regenerated Shoots and Transient Expression in Morphogenic Explants in Prunus avium cultivar 'Burlat C1' / M.C. Piagnani, R. Chiozzotto. - In: EUROPEAN JOURNAL OF HORTICULTURAL SCIENCE. - ISSN 1611-4426. - 75:3(2010 Mar), pp. 132-138.
Abstract:
Biotechnology may represent alternative ways to
genetic improvement of sweet cherry exploiting somaclonal
variation and genetic transformation. In this
paper a method to induce efficient shoot regeneration
from somatic explants of ‘Burlat C1’ is reported:
52.0 % caulogenesis was obtained from basal callus
formed after culturing shoot apical portions in the
dark. Considering the significant onset of adventitious
shoots showing variegated leaves their in vitro performances
have been evaluated as compared to green
(normal) shoots. The influence of cefotaxime, cefotaxime
plus vancomycin and timentin was assessed both
for decontamination and regeneration efficiency prior
transforming morphogenic masses with A. tumefaciens
(C58C1 pDN3514), carrying the rolABC genes from A.
rhizogenes, p35GUS-intron visible marker and nptII
antibiotic resistance gene. GUS test indicated the presence
of transient expression in shoot primordia nevertheless
it was not possible to recover transgenic shoots
probably due to the formation of chimeric meristems
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
A.tumefaciens ; acetosyringone ; cefotaxime ; leaf variegation ; timentin ; vancomycin
Elenco autori:
M.C. Piagnani, R. Chiozzotto
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