Short-Term Exposure to Benzo(a)Pyrene Causes Disruption of GnRH Network in Zebrafish Embryos
Articolo
Data di Pubblicazione:
2023
Citazione:
Short-Term Exposure to Benzo(a)Pyrene Causes Disruption of GnRH Network in Zebrafish Embryos / I. Gentile, V. Vezzoli, S. Martone, M.G. Totaro, M. Bonomi, L. Persani, F. Marelli. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1661-6596. - 24:8(2023), pp. 6913.1-6913.10. [10.3390/ijms24086913]
Abstract:
Benzo(a)pyrene (BaP), a polycyclic aromatic hydrocarbon, is considered a common endocrine disrupting chemical (EDC) with mutagenic and carcinogenic effects. In this work, we evaluated the effects of BaP on the hypothalamo-pituitary-gonadal axis (HPG) of zebrafish embryos. The embryos were treated with 5 and 50 nM BaP from 2.5 to 72 hours post-fertilization (hpf) and obtained data were compared with those from controls. We followed the entire development of gonadotropin releasing hormone (GnRH3) neurons that start to proliferate from the olfactory region at 36 hpf, migrate at 48 hpf and then reach the pre-optic area and the hypothalamus at 72 hpf. Interestingly, we observed a compromised neuronal architecture of the GnRH3 network after the administration of 5 and 50 nM BaP. Given the toxicity of this compound, we evaluated the expression of genes involved in antioxidant activity, oxidative DNA damage and apoptosis and we found an upregulation of these pathways. Consequently, we performed a TUNEL assay and we confirmed an increment of cell death in brain of embryos treated with BaP. In conclusion our data reveal that short-term exposure of zebrafish embryos to BaP affects GnRH3 development likely through a neurotoxic mechanism.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
benzo(a)pyrene (BaP); endocrine disruptor chemical (EDC); gonadotropin releasing hormone (GnRH); hypolamo-pituitary-gonadal (HPG) axis; zebrafish
Elenco autori:
I. Gentile, V. Vezzoli, S. Martone, M.G. Totaro, M. Bonomi, L. Persani, F. Marelli
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