Molecular characterization of three novel splicing mutations causing factor V deficiency and analysis of the F5 gene splicing pattern
Articolo
Data di Pubblicazione:
2008
Citazione:
Molecular characterization of three novel splicing mutations causing factor V deficiency and analysis of the F5 gene splicing pattern / C. Dall'Osso, I. Guella, S. Duga, N. Locatelli, E.M. Paraboschi, M. Spreafico, A. Afrasiabi, C. Pechlaner, F. Peyvandi, M.L. Tenchini, R. Asselta. - In: HAEMATOLOGICA. - ISSN 0390-6078. - 93:10(2008 Oct), pp. 1505-1513.
Abstract:
BACKGROUND: Factor V deficiency is a rare autosomal recessive hemorrhagic disorder, associated with bleeding manifestations of variable severity. In the present study, we investigated the molecular basis of factor V deficiency in three patients, and performed a comprehensive analysis of the factor V gene (F5) splicing pattern. DESIGN AND METHODS: Mutational screening was performed by DNA sequencing. Wild-type and mutant F5 mRNA were expressed by transient transfection in COS-1 cells, followed by reverse-transcriptase polymerase chain reaction and sequencing. Real-time reverse-transcriptase polymerase chain reaction was used to evaluate degradation of mRNA carrying premature termination codons. RESULTS: Mutational screening identified three hitherto unknown splicing mutations (IVS8+6T>C, IVS21+1G>A, and IVS24+1_+4delGTAG). Production of mutant transcripts in COS-1 cells demonstrated that both IVS21+1G>A and IVS24+1_+4delGTAG cause the activation of cryptic donor splice sites, whereas IVS8+6T>C causes exon-8 skipping (F5-Delta 8-mRNA). Interestingly, F5-Delta 8-mRNA was also detected in wild-type transfected samples, human liver, platelets, and HepG2 cells, demonstrating that F5 exon-8 skipping takes place physiologically. Since F5-Delta 8-mRNA bears a premature termination codons, we investigated whether this transcript is subjected to nonsense-mediated mRNA decay degradation. The results confirmed the involvement of nonsense-mediated mRNA decay in the degradation of F5 PTC(+) mRNA. Moreover, a comprehensive analysis of the F5 splicing pattern led to the identification of two in-frame splicing variants resulting from skipping of exons 3 and 5-6. CONCLUSIONS: The functional consequences of three splicing mutations leading to FV deficiency were elucidated. Furthermore, we report the identification of three alternatively spliced F5 transcripts.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
C. Dall'Osso, I. Guella, S. Duga, N. Locatelli, E.M. Paraboschi, M. Spreafico, A. Afrasiabi, C. Pechlaner, F. Peyvandi, M.L. Tenchini, R. Asselta
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