Genetic dissection of fruit maturity date in apricot (P. armeniaca L.) through a Single Primer Enrichment Technology (SPET) approach
Articolo
Data di Pubblicazione:
2022
Citazione:
Genetic dissection of fruit maturity date in apricot (P. armeniaca L.) through a Single Primer Enrichment Technology (SPET) approach / I. Baccichet, R. Chiozzotto, D. Scaglione, D. Bassi, L. Rossini, M. Cirilli. - In: BMC GENOMICS. - ISSN 1471-2164. - 23:1(2022), pp. 712.1-712.16. [10.1186/s12864-022-08901-1]
Abstract:
Background: Single primer enrichment technology (SPET) is an emerging and increasingly popular solution for
high-throughput targeted genotyping in plants. Although SPET requires a priori identification of polymorphisms for
probe design, this technology has potentially higher reproducibility and transferability compared to other reduced
representation sequencing (RRS) approaches, also enabling the discovery of closely linked polymorphisms surrounding
the target one.
Results: The potential for SPET application in fruit trees was evaluated by developing a 25K target SNPs assay to
genotype a panel of apricot accessions and progenies. A total of 32,492 polymorphic sites were genotyped in 128
accessions (including 8,188 accessory non-target SNPs) with extremely low levels of missing data and a significant
correlation of allelic frequencies compared to whole-genome sequencing data used for array design. Assay performance
was further validated by estimating genotyping errors in two biparental progenies, resulting in an overall 1.8%
rate. SPET genotyping data were used to infer population structure and to dissect the architecture of fruit maturity
date (MD), a quantitative reproductive phenological trait of great agronomical interest in apricot species. Depending
on the year, GWAS revealed loci associated to MD on several chromosomes. The QTLs on chromosomes 1 and 4 (the
latter explaining most of the phenotypic variability in the panel) were the most consistent over years and were further
confirmed by linkage mapping in two segregating progenies.
Conclusions: Besides the utility for marker assisted selection and for paving the way to in-depth studies to clarify the
molecular bases of MD trait variation in apricot, the results provide an overview of the performance and reliability of
SPET for fruit tree genetics.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Apricot, Fruit, Fruit crops genomics, GWAS, Maturity date, SPET
Elenco autori:
I. Baccichet, R. Chiozzotto, D. Scaglione, D. Bassi, L. Rossini, M. Cirilli
Link alla scheda completa:
Link al Full Text: