The lipid moiety of haemozoin (Malaria Pigment) and P. falciparum parasitised red blood cells bind synthetic and native endothelin-1
Articolo
Data di Pubblicazione:
2010
Citazione:
The lipid moiety of haemozoin (Malaria Pigment) and P. falciparum parasitised red blood cells bind synthetic and native endothelin-1 / N. Basilico, S. Parapini, F. Sisto, M.F. Omodeo-Salè, P. Coghi, F. Ravagnani, P. Olliaro, D. Taramelli. - In: JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY. - ISSN 1110-7243. - 2010:Art. num. 854927(2010), pp. 854927.1-854927.9. [10.1155/2010/854927]
Abstract:
Endothelin1 (ET-1) is a 21-amino acid peptide produced by the vascular endothelium under hypoxia, that acts locally as regulator of vascular tone and inflammation. The role of ET-1 in Plasmodium falciparum malaria is unknown, although tissue hypoxia is frequent as a result of the cytoadherence of parasitized red blood cell (pRBC) to the microvasculature. Here, we show that both synthetic and endothelial-derived ET-1 are removed by parasitized RBC (D10 and W2 strains, chloroquine sensitive, and resistant, resp.) and native haemozoin (HZ, malaria pigment), but not by normal RBC, delipidized HZ, or synthetic beta-haematin (BH). The effect is dose dependent, selective for ET-1, but not for its precursor, big ET-1, and not due to the proteolysis of ET-1. The results indicate that ET-1 binds to the lipids moiety of HZ and membranes of infected RBCs. These findings may help understanding the consequences of parasite sequestration in severe malaria.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
N. Basilico, S. Parapini, F. Sisto, M.F. Omodeo-Salè, P. Coghi, F. Ravagnani, P. Olliaro, D. Taramelli
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