Urinary polycyclic aromatic hydrocarbons and monohydroxy metabolites as biomarkers of exposure in coke-oven workers
Articolo
Data di Pubblicazione:
2009
Citazione:
Urinary polycyclic aromatic hydrocarbons and monohydroxy metabolites as biomarkers of exposure in coke-oven workers / F. Rossella, L. Campo, S. Pavanello, L. Kapka, E. Siwinska, S. Fustinoni. - In: OCCUPATIONAL AND ENVIRONMENTAL MEDICINE. - ISSN 1351-0711. - 66:8(2009), pp. 509-516.
Abstract:
Objectives: To assess exposure to polycyclic aromatic hydrocarbons (PAHs) using 13 unmetabolised PAHs (UPAHs) and 12 monohydroxy metabolites (OHPAHs) in urine, and to compare the utility of these biomarkers.
Methods: 55 male Polish coke oven workers collected urine spot samples after a workshift. U-PAHs (naphthalene,
acenaphtylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo[a]anthracene, chrysene, benzo[k]fluoranthene, benzo[b]fluoranthene, benzo[a]pyrene) were determined by automatic solid phase micro-extraction followed by gas
chromatography/mass spectrometry (GC/MS). OHPAHs (1- and 2-hydroxynaphthalene, 2- and 9-hydroxyfluorene,4-, 9-, 3-, 1- and 2-hydroxyphenanthrene, 1-hydroxypyrene,
6-hydroxychrysene, 3-hydroxybenzo[a]pyrene) were determined, after liquid/liquid extraction and derivatisation,
by GC/MS.
Results: U-PAHs from naphthalene to chrysene were found in 100% of samples, and heavier U-PAHs in 7–22% of samples. OHPAHs up to 1-hydroxypyrene were found in 100% of samples, while 6-hydroxychrysene and 3-
hydroxybenzo[a]pyrene were always below the quantification limit. Median naphthalene, phenanthrene, pyrene, chrysene and benzo[a]anthracene levels were 0.806,0.721, 0.020, 0.032 and 0.035 mg/l, while hydroxynaphthalenes,hydroxyphenanthrenes and 1-hydroxypyrene levels were 81.1, 18.9 and 15.4 mg/l. For each chemical, the ratio between U-PAH and the corresponding OHPAH ranged from 1:26 to 1:1000. Significant correlations between logged values of U-PAHs and OHPAHs,between U-PAHs, and between OHPAHs were found,with Pearson’s r ranging from 0.27 to 0.97.
Conclusion: Current analytical techniques allow specific and simultaneous measurement of several urinary determinants of PAHs in humans. The results of these measurements support the use of U-PAHs as biomarkers
of exposure and suggest the spectrum of chemicals to be investigated, including carcinogenic chrysene and benzo[a]anthracene, should be widened.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
polycyclic aromatic hydrocarbons; urine; coke-oven workers
Elenco autori:
F. Rossella, L. Campo, S. Pavanello, L. Kapka, E. Siwinska, S. Fustinoni
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