Ternary complex formation between MADS-box transcription factors and the histone fold protein NF-YB
Articolo
Data di Pubblicazione:
2002
Citazione:
Ternary complex formation between MADS-box transcription factors and the histone fold protein NF-YB / S. Masiero, C. Imbriano, F. Ravasio, R. Favaro, N. Pelucchi, M. Sari Gorla, R. Mantovani, L. Colombo, M. Kater. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 277:29(2002 Jul 19), pp. 26429-26435.
Abstract:
MADS-box proteins are transcription factors present in different eukaryotic kingdoms. In contrast to plants, for mammalian and yeast MADS-box proteins ternary complex formation with unrelated transcription factors was reported. We show here the first identification of such ternary interaction in plants. A rice seed-specific NF-YB was identified as partner of OsMADS18 by two-hybrid screening. NF-YB contains a histone fold motif, HFM,(1) and is part of the trimeric CCAAT-binding NF-Y complex. OsMADS18, alone or in combination with a natural partner, interacts with OsNF-YB1 through the MADS and I regions. The mouse NF-YB also associates with OsMADS18 in vivo and in vitro as a NF-YB-NF-YC dimer. Other rice MADS-box proteins do not interact in these assays, indicating specificity for the interaction. OsNF-YB1 is capable of heterodimerizing with NF-YC, but not trimerizing with NF-YA, thus precluding CCAAT binding. Mutation of the variant Asp at position 99 of the HFM alpha2-helix into a conserved serine recovers the capacity to interact with NF-YA, but not with DNA. This is the first indication that members of the NF-YB family work through mechanisms independent of the CCAAT box.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
FLOWER DEVELOPMENT ; HOMEOTIC GENES ; ANTIRRHINUM-MAJUS ; OVULE DEVELOPMENT ; RNA-POLYMERASE ; HOST STRAIN ; CCAAT BOXES ; ARABIDOPSIS ; DOMAIN ; FAMILY
Elenco autori:
S. Masiero, C. Imbriano, F. Ravasio, R. Favaro, N. Pelucchi, M. Sari Gorla, R. Mantovani, L. Colombo, M. Kater
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