Data di Pubblicazione:
2020
Citazione:
Human immunoglobulin G responses to Cimex lectularius L. saliva / J.M. Sheele, B. Ferrari, J. Goddard, D. Schlatzer, K.C. Lundberg, K. Guinto, M.E. Embers, A.B. Young, G.E. Ridge, G. Damiani, T.S. McCormick. - In: PARASITE IMMUNOLOGY. - ISSN 0141-9838. - 42:12(2020), pp. e12764.1-e12764.7. [10.1111/pim.12764]
Abstract:
Aims: To investigate the immunoglobulin (Ig) G response after being fed upon by Cimex lectularius L. Methods and Results: Participants were fed upon by three male C lectularius insects weekly for a month. Blood was obtained before the feeding and at the last feeding, which was used for immunoblots against bed bug salivary gland extract, with antihuman Immunoglobulin G (IgG) secondary antibodies. No consistent IgG changes developed in 11 humans serially fed upon by C lectularius. Two participants had new IgG responses to proteins at molecular weights of approximately 12-13 kDa, and one had an IgG response to a protein at approximately 40 kDa. At the last study visit, more intense IgG bands to proteins at molecular weights of 12-13 kDa had developed in 55% of participants (6/11) and at molecular weights of ≈30, ≈40 and ≈70 kDa in 45% (5/11) compared with the first study visit. Nitrophorin and apyrase were the most common C lectularius proteins identified with liquid chromatography-tandem mass spectrometry in both crushed bed bug salivary gland extract and post–bed bug feeding extract. Conclusions: Human participants did not have consistent IgG responses to crushed C lectularius salivary gland extract.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
antibody; bed bug; Cimex lectularius; cimicosis; IgG; immunoglobulin; saliva
Elenco autori:
J.M. Sheele, B. Ferrari, J. Goddard, D. Schlatzer, K.C. Lundberg, K. Guinto, M.E. Embers, A.B. Young, G.E. Ridge, G. Damiani, T.S. Mccormick
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