Extra Virgin Olive Oil Phenol Extracts Exert Hypocholesterolemic Effects through the Modulation of the LDLR Pathway: In Vitro and Cellular Mechanism of Action Elucidation
Articolo
Data di Pubblicazione:
2020
Citazione:
Extra Virgin Olive Oil Phenol Extracts Exert Hypocholesterolemic Effects through the Modulation of the LDLR Pathway: In Vitro and Cellular Mechanism of Action Elucidation / C. Lammi, M. Bellumori, L. Cecchi, M. Bartolomei, C. Bollati, M.L. Clodoveo, F. Corbo, A. Arnoldi, N. Mulinacci. - In: NUTRIENTS. - ISSN 2072-6643. - 12:6(2020 Jun 09). [10.3390/nu12061723]
Abstract:
This study was aimed at investigating the hypocholesterolemic eects of extra virgin
olive oil (EVOO) phenols and the mechanisms behind the eect. Two phenolic extracts were
prepared from EVOO of dierent cultivars and analyzed using the International Olive Council (IOC)
ocial method for total phenols, a recently validated hydrolytic procedure for total hydroxytyrosol
and tyrosol, and 1H-NMR analysis in order to assess their secoiridoid profiles. Both of the extracts
inhibited in vitro the 3-hydroxy-3-methylglutaryl co-enzyme A reductase (HMGCoAR) activity in
a dose-dependent manner. After the treatment of human hepatic HepG2 cells (25 g/mL), they
increased the low-density lipoprotein (LDL) receptor protein levels through the activation of the sterol
regulatory element binding proteins (SREBP)-2 transcription factor, leading to a better ability of HepG2
cells to uptake extracellular LDL molecules with a final hypocholesterolemic eect. Moreover, both of
the extracts regulated the intracellular HMGCoAR activity through the increase of its phosphorylation
by the activation of AMP-activated protein kinase (AMPK)-pathways. Unlike pravastatin, they did
not produce any unfavorable eect on proprotein convertase subtilisin/kexin 9 (PCSK9) protein
level. Finally, the fact that extracts with dierent secoiridoid profiles induce practically the same
biological eects suggests that the hydroxytyrosol and tyrosol derivatives may have similar roles in
hypocholesterolemic activity.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
EVOO phenols; HepG2 cell; hypocholesterolemic; PCSK9; LD; receptor
Elenco autori:
C. Lammi, M. Bellumori, L. Cecchi, M. Bartolomei, C. Bollati, M.L. Clodoveo, F. Corbo, A. Arnoldi, N. Mulinacci
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