Influence of the culture conditions on extracellular lyase activity related to K5 polysaccharide
Articolo
Data di Pubblicazione:
2000
Citazione:
Influence of the culture conditions on extracellular lyase activity related to K5 polysaccharide / M. Manzoni, S. Bergomi, M. Rollini. - In: BIOTECHNOLOGY LETTERS. - ISSN 0141-5492. - 22:1(2000), pp. 81-85.
Abstract:
The K5 capsular polysaccharide antigen of some Escherichia coli strains is the non-sulphated precursor in heparin biosynthesis. It is composed by two components, 16 000 and 1500 Da, whose ratio depends on the activity of the extracellular form of a lyase synthesized by the same K5 producer strain. The lyase activity on the K5 chain size was greatly influenced by the medium composition employed for the lyase production. The control of lyase activity results in defined ratios of the two components in the K5 polysaccharide that is suitable for the semisynthetic production of heparin-like molecules.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Escherichia coli; Extracellular lyase; Heparin-like; Polysaccharide
Elenco autori:
M. Manzoni, S. Bergomi, M. Rollini
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