BPC transcription factors and a Polycomb Group protein confine the expression of the ovule identity gene SEEDSTICK in Arabidodpsis
Articolo
Data di Pubblicazione:
2020
Citazione:
BPC transcription factors and a Polycomb Group protein confine the expression of the ovule identity gene SEEDSTICK in Arabidodpsis / R. PETRELLA, F. CASELLI, I. ROIG VILLANOVA, V. Vignati, M. CHIARA, E. Ignacio, L. TADINI, M. KATER, V. GREGIS. - In: PLANT JOURNAL. - ISSN 0960-7412. - (2020 Jan 06). [Epub ahead of print] [10.1111/tpj.14673]
Abstract:
The BASIC PENTACYSTEINE (BPC) GAGA (C-box) binding proteins belong to a small plant transcription factor family. We previously reported that BPCs of class I bind directly to C-boxes in the SEEDSTICK (STK) promoter and the mutagenesis of these cis-elements affects STK expression in the flower. The MADS-domain factor SHORT VEGETATIVE PHASE (SVP) is another key regulator of STK. Direct binding of SVP to CArG-boxes in the STK promoter are required to repress its expression during the first stages of flower development. Here we show that BPCs of class II directly interact with SVP and that MADS-domain binding sites in the STK promoter region are important for the correct spatial and temporal expression of this homeotic gene. Furthermore, we show that BPCs of class I and II act redundantly to repress STK expression in the flower, most likely by recruiting TERMINAL FLOWER 2/LIKE HETEROCHROMATIN PROTEIN 1 (TFL2/LHP1) and mediating the establishment and the maintenance of H3K27me3 repressive marks on the DNA. We investigate the role of LHP1 in the regulation of STK expression. Besides providing a better understanding of the role of BPC transcription factors in the regulation of STK expression, our results suggest the existence of a more general regulatory complex composed of BPCs, MADS-domain factors and PRCs, that cooperate to regulate gene expression in reproductive tissues. We believe that our data along with the molecular model herein described could provide significant insights for a more comprehensive understanding of gene regulation in plants.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
MADS-box; BPCs; homeotic genes; STK; LHP1; PRC; Arabidopsis thaliana; transcription factors
Elenco autori:
R. PETRELLA, F. CASELLI, I. ROIG VILLANOVA, V. Vignati, M. CHIARA, E. Ignacio, L. TADINI, M. KATER, V. GREGIS
Link alla scheda completa:
Link al Full Text: