Flow-based biocatalysis : application to peracetylated arabinofuranosyl-1,5-arabinofuranose synthesis
Articolo
Data di Pubblicazione:
2018
Citazione:
Flow-based biocatalysis : application to peracetylated arabinofuranosyl-1,5-arabinofuranose synthesis / T. Bavaro, A. Pinto, F. Dall'Oglio, M.J. Hernaiz, C.F. Morelli, P. Zambelli, C. De Micheli, P. Conti, L. Tamborini, M. Terreni. - In: PROCESS BIOCHEMISTRY. - ISSN 1359-5113. - 72(2018), pp. 112-118.
Abstract:
The lipase-catalyzed regioselective hydrolysis of peracetylated arabinose was performed in a packed bed flow reactor (PBR). In particular, the hydrolysis of the α anomer of peracetylated arabinose catalyzed by Novozym 435 resulted in the monodeprotection of C5 in only 5 min and 91% yield. By using the immobilized Pseudomonas stutzeri lipase, the regioselective hydrolysis of the β anomer was also accomplished affording the C1 deacetylated deriv. in 30 min with good yields. The high local concn. of the immobilized biocatalyst in the PBR allow for a significant redn. of the reaction time; moreover, repeated re-use, and easy downstream processing increase the efficiency and the productivity of the process, if compared to the classical batch procedure. In fact, under optimized conditions, the specific reaction rate of the biocatalyzed flow reaction showed an increase of more than 300 times compared to the batch one. The obtained building blocks were prepd. in gram scale and then used for the synthesis of peracetylated arabinofuranosyl-1,5-arabinofuranose. [on SciFinder(R)]
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
lipases; enzymatic hydrolysis; flow chemistry; immobilized enzymes; disaccharides; tubercolosis
Elenco autori:
T. Bavaro, A. Pinto, F. Dall'Oglio, M.J. Hernaiz, C.F. Morelli, P. Zambelli, C. De Micheli, P. Conti, L. Tamborini, M. Terreni
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