Data di Pubblicazione:
2017
Citazione:
Epidermal barrier reaction to an in vitro psoriatic microenvironment / E. Donetti, L. Cornaghi, F. Arnaboldi, F. Ricceri, L. Pescitelli, M. Maiocchi, F. Carriero, F. Baruffaldi Preis, F. Prignano. - In: EXPERIMENTAL CELL RESEARCH. - ISSN 0014-4827. - 15:2(2017 Nov 15), pp. 180-188. [10.1016/j.yexcr.2017.09.004]
Abstract:
Keratinocytes (KCs) and Langerhans cells (LCs) contribute to create the epidermal barrier. To form a functional epidermis, KCs express filaggrin and Toll-like Receptors (TLRs). LCs are the first line of epidermal defence and can be activated by interleukin (IL)-17 and Tumor Necrosis Factor (TNF)-alpha. In psoriasis, an alteration of TLR expression, a defective expression of filaggrin, and LC activation occur. In organotypic cultures of human skin we investigated the interplay between IL-17 and TNF-alpha on i) expression of filaggrin, TLR2, 7 and 9, and Nuclear Factor (NF)-kB localization by immunofluorescence and ii) LC ultrastructural features by transmission electron microscopy. Normal human skin was obtained after aesthetic surgery (n=7), overnight incubated in a Transwell system, and exposed to TNF-alpha and/or IL-17 for 24 (T24), 48 (T48), and 72 (T72) hours. Cytokines always influenced the expression of filaggrin. TNF-alpha alone activated LCs only starting from T48. TLR2 and TLR7 expressions were affected at T24 by IL-17 and the combination of cytokines, but not by TNF-alpha. TLR9-positive cells were detectable in the granular layer after cytokine exposure. A nuclear localization of NF-kB was always observed after cytokine incubation. In conclusion, each cytokine possess an intrinsic activity on the different components of the epidermal barrier.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
TLRs; transmission electron microscopy; immunofluorescence; innate immunity; nuclear factor kappa B; langerhans cells
Elenco autori:
E. Donetti, L. Cornaghi, F. Arnaboldi, F. Ricceri, L. Pescitelli, M. Maiocchi, F. Carriero, F. Baruffaldi Preis, F. Prignano
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