In vitro sensitivity to methyl-prednisolone is associated with clinical response in pediatric idiopathic nephrotic syndrome
Articolo
Data di Pubblicazione:
2016
Citazione:
In vitro sensitivity to methyl-prednisolone is associated with clinical response in pediatric idiopathic nephrotic syndrome / E. Cuzzoni, S. De Iudicibus, G. Stocco, D. Favretto, M. Pelin, G. Messina, L. Ghio, E. Monti, A. Pasini, G. Montini, G. Decorti. - In: CLINICAL PHARMACOLOGY & THERAPEUTICS. - ISSN 1532-6535. - 100:3(2016), pp. 268-274. [10.1002/cpt.372]
Abstract:
The aim of this study was to evaluate the in vitro steroid sensitivity as predictor of clinical response to glucocorticoids in childhood idiopathic nephrotic syndrome (INS). Seventy-four patients (median age 4.33, IQR 2.82-7.23; 63.5% male) were enrolled in a prospective multicenter study: in vitro steroid inhibition of patients' peripheral blood mononuclear cell proliferation was evaluated by [methyl-3 H] thymidine incorporation assay at disease onset (T0) and after 4 weeks (T4) of treatment. Steroid dependence was associated with increased in vitro sensitivity at T4 assessed both as drug concentration inducing 50% of inhibition (IC50 ; OR=0.48, 95%CI=0.24-0.85; p-value=0.0094) and maximum inhibition at the highest drug concentration (Imax ; OR=1.13, 95%CI=1.02-1.31; p-value=0.017). IC50 > 4.4nM and Imax < 92% at T4 were good predictors for optimal clinical response. These results suggest that this test may be useful for predicting the response to glucocorticoid therapy in pediatric INS.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Idiopathic nephrotic syndrome; in vitro sensitivity; peripheral blood mononuclear cells; steroid response
Elenco autori:
E. Cuzzoni, S. De Iudicibus, G. Stocco, D. Favretto, M. Pelin, G. Messina, L. Ghio, E. Monti, A. Pasini, G. Montini, G. Decorti
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