Interleukin 22 early affects keratinocyte differentiation, but not proliferation, in a three-dimensional model of normal human skin
Articolo
Data di Pubblicazione:
2016
Citazione:
Interleukin 22 early affects keratinocyte differentiation, but not proliferation, in a three-dimensional model of normal human skin / E. Donetti, L. Cornaghi, F. Arnaboldi, L. Landoni, P. Romagnoli, N. Mastroianni, L. Pescitelli, F.W. Baruffaldi Preis, F. Prignano. - In: EXPERIMENTAL CELL RESEARCH. - ISSN 0014-4827. - 345:2(2016 Jul 15), pp. 247-254. [10.1016/j.yexcr.2016.05.004]
Abstract:
Interleukin (IL)-22 is a pro-inflammatory cytokine driving the progression of the psoriatic lesion with other cytokines, as Tumor Necrosis Factor (TNF)-alpha and IL-17. Our study was aimed at evaluating the early effect of IL-22 alone or in combination with TNF-alpha and IL-17 by immunofluorescence on i) keratinocyte (KC) proliferation, ii) terminal differentiation biomarkers as keratin (K) 10 and 17 expression,
iii) intercellular junctions. Transmission electron microscopy (TEM) analysis was performed. A model of human skin culture reproducing a psoriatic microenvironment was used. Plastic surgery explants were obtained from healthy young women (n¼7) after informed consent. Fragments were divided before adding IL-22 or a combination of the three cytokines, and harvested 24 (T24), 48 (T48), and 72 (T72) h later. From T24, in IL-22 samples we detected a progressive decrease in K10 immunostaining in the spinous layer paralleled by K17 induction. By TEM, after IL-22 incubation, keratin aggregates were evident in the perinuclear area. Occludin immunostaining was not homogeneously distributed. Conversely, KC proliferation was not inhibited by IL-22 alone, but only by the combination of cytokines. Our results suggest that IL-22 affects keratinocyte terminal differentiation, whereas, in order to induce a proliferation impairment, a more complex psoriatic-like microenvironment is needed.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Keratin 10; Keratin 17; transmission electron microscopy; Interleukin-17; tumor necrosis factor alpha
Elenco autori:
E. Donetti, L. Cornaghi, F. Arnaboldi, L. Landoni, P. Romagnoli, N. Mastroianni, L. Pescitelli, F.W. Baruffaldi Preis, F. Prignano
Link alla scheda completa: