Data di Pubblicazione:
1989
Citazione:
Yeast 2 micron vectors replicate and undergo recombination in Torulaspora delbrueckii / C. Compagno, B.M. Ranzi, E. Martegani. - In: MOLECULAR MICROBIOLOGY. - ISSN 0950-382X. - 3:8(1989 Aug), pp. 1003-1010.
Abstract:
In order to develop a procedure for transformation of the industrial yeast Torulaspora delbrueckii, we have constructed a set of recombinant plasmids carrying Saccharomyces cerevisiae ARS and 2 microns origin of replication and kanamycin-G418 resistance gene of Tn903(601) as a selective marker. In this paper we show that S. cerevisiae ARS vectors can replicate autonomously and that vectors bearing the whole S. cerevisiae 2 microns sequence yield stable transformants. We also present evidence to show that 2 microns vectors undergo an FLP-mediated inter- and intramolecular recombination, which suggests that T. delbrueckii can support the amplification and partition mechanisms of these plasmids.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Blotting, Southern; Gene Expression; Lac Operon; Plasmids; Promoter Regions, Genetic; Restriction Mapping; Saccharomyces; Saccharomyces cerevisiae; Genetic Vectors; Recombination, Genetic; Transformation, Genetic
Elenco autori:
C. Compagno, B.M. Ranzi, E. Martegani
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