Data di Pubblicazione:
2015
Citazione:
Enhanced amino acid utilization sustains growth of cells lacking Snf1/AMPK / R. Nicastro, F. Tripodi, C. Guzzi, V. Reghellin, S. Khoomrung, C. Capusoni, C. Compagno, C. Airoldi, J. Nielsen, L. Alberghina, P. Coccetti. - In: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. - ISSN 0167-4889. - 1853:7(2015), pp. 1615-1625. [10.1016/j.bbamcr.2015.03.014]
Abstract:
The metabolism of proliferating cells shows common features even in evolutionary distant organisms such as mammals and yeasts, for example the requirement for anabolic processes under tight control of signaling pathways. Analysis of the rewiring of metabolism, which occurs following the dysregulation of signaling pathways, provides new knowledge about the mechanisms underlying cell proliferation.The key energy regulator in yeast Snf1 and its mammalian ortholog AMPK have earlier been shown to have similar functions at glucose limited conditions and here we show that they also have analogies when grown with glucose excess. We show that loss of Snf1 in cells growing in 2% glucose induces an extensive transcriptional reprogramming, enhances glycolytic activity, fatty acid accumulation and reliance on amino acid utilization for growth. Strikingly, we demonstrate that Snf1/AMPK-deficient cells remodel their metabolism fueling mitochondria and show glucose and amino acids addiction, a typical hallmark of cancer cells.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Budding yeast; Gene chip; Glucose; Metabolism; Respiration; Saccharomyces cerevisiae; AMP-Activated Protein Kinases; Adenosine Triphosphate; Amino Acids; Biocatalysis; Carbon; Cell Proliferation; Cellular Reprogramming; Citric Acid Cycle; Fatty Acids; Fermentation; Gene Deletion; Gene Expression Regulation, Fungal; Genes, Fungal; Glucose; Glutamic Acid; Glycolysis; Models, Biological; Oxidative Phosphorylation; Protein-Serine-Threonine Kinases; Saccharomyces cerevisiae; Transcription, Genetic; Up-Regulation; Cell Biology; Molecular Biology; Medicine (all)
Elenco autori:
R. Nicastro, F. Tripodi, C. Guzzi, V. Reghellin, S. Khoomrung, C. Capusoni, C. Compagno, C. Airoldi, J. Nielsen, L. Alberghina, P. Coccetti
Link alla scheda completa: