Quality control of plant food supplements containing Citrus aurantium L. : a new HPLC method to measure active amines
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Data di Pubblicazione:
2014
Citazione:
Quality control of plant food supplements containing Citrus aurantium L. : a new HPLC method to measure active amines / C. Di Lorenzo, A. Dos Santos, F. Colombo, G. Frigerio, E. Moro, P. Restani. ((Intervento presentato al 10. convegno National Congress of Food Chemistry - CHIMALI tenutosi a Firenze nel 2014.
Abstract:
Botanicals and botanical derivatives are widely used in Western diets from various
sources; among them, plant food supplements (PFS) are the most usual
commercialized forms. Citrus aurantium L. (bitter orange) is frequently used as an
ingredient of food supplements aimed to reduce body weight or improve general
physical performances. The most important compounds of C. aurantium are amines
having adrenergic activity: octopamine, synephrine, tyramine, N-methyl-tyramine
and hordenine. The C. aurantium extract is generally allowed in food supplements,
but, in several countries, limits have been established, since some cases of adverse
effects, involving mainly the cardiovascular system, have been reported in the
scientific literature (1). With the decree dated July 09 2012, the Italian Ministry of
Health established a limit of synephrine in C.aurantium-based products, in order to
maintain the daily intake < 30 mg, whereas the sum of other amines, including
octopamine, should be lower than 1/8 (12.5%) synephrine (2). On these bases, quality
control of PFS is of crucial importance also taking into consideration that the illicit
use of C. aurantium and/or its synthetic amines is not infrequent. The aim of this
work was to set up and validate a new, quick and simple HPLC method using a
fluorimetric detection; this method can be easily applied to the quality control of
PFS containing C. aurantium, offering a new tool for industries involved in PFS
production and laboratories responsible for the post-marketing control.
1. Haller, C.A., Benowitz, N.L. (2000). Adverse cardiovascular and central nervous
system events associated with dietary supplements containing ephedra alkaloids.
The New England Journal of Medicine, 343, 1833-1838.
2. Italian Ministry of Health (2002). Circolare n. 3. Applicazione della procedura di
notifica di etichetta di cui all'art. 7 del decreto legislativo n. 111/1992, ai prodotti a
base di piante e derivati aventi finalità salutistiche In Gazzetta Ufficiale n.188,
08.12.2002.
The research leading to these results has received funding from the European
Community’s Seventh Framework Program (FP7/2007-2013) under grant
agreement n° 245199. It has been carried out within the PlantLIBRA project
(website: www.plantlibra.eu). This report does not necessarily reflect the
Commission's views or its future policy on this area.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
C. Di Lorenzo, A. Dos Santos, F. Colombo, G. Frigerio, E. Moro, P. Restani
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