Data di Pubblicazione:
2014
Citazione:
Legume proteins : ACE-inhibitory activity of enzymatic hydrolysates / G. Boschin, G.M. Scigliuolo, D. Resta, A. Arnoldi - In: XXV Congresso Nazionale della Società Chimica Italiana : atti del congresso[s.l] : Società Chimica Italiana, 2014 Sep 07. - pp. 341-341 (( Intervento presentato al 25. convegno Congresso Nazionale Società Chimica Italiana (SCI) tenutosi a Arcavacata di Rende nel 2014.
Abstract:
The enzymatic hydrolysis of legume proteins produce peptide mixtures able to inhibit angiotensin-converting enzyme (ACE), that is involved in the renin-angiotensin system for the regulation of blood pressure [1].
In this work ACE-inhibitory activity of hydrolysates obtained by pepsin digestion of proteins of some legumes, such as chickpea, common bean, lentil, lupin, pea, and soybean were investigated, by using the tripeptide hippuryl-histidyl-leucine (HHL), as model peptide, and HPLC-DAD, as analytical method. Soybean and lupin resulted the most promising ones, with IC50 values of 224 and 226 µg/ml, respectively [2].
Moreover three different lupin species (Lupinus albus, L. angustifolius, L. luteus) were studied with the final goal of selecting the best enzyme/species combination for an efficient production of ACE-inhibitory peptide mixtures. Different enzymes were tested: trypsin, chymotrypsin, corolase PP, umamizyme, and flavourzyme. Pepsin gave peptides with the best activity followed by pepsin + trypsin, chymotrypsin, trypsin, corolase PP, umamizyme, and flavourzyme [3]. This investigation confirms that lupin proteins may be a source of ACE-inhibitory peptides, and foresee the application of lupin proteins into functional foods or dietary supplements.
[1] F. Roy, J.I. Boye, and B.K. Simpson, B. K. Food Res. Int. 2010, 43, 432-442.
[2] G. Boschin, G.M. Scigliuolo, D. Resta and A. Arnoldi Food Chem., 2014, 145, 34-40.
[3] G. Boschin, G.M. Scigliuolo, D. Resta and A. Arnoldi J. Agric. Food Chem. 2014, 62, 1846-1851.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
lupin; hypertension; plant protein; cardiovascular disease
Elenco autori:
G. Boschin, G.M. Scigliuolo, D. Resta, A. Arnoldi
Link alla scheda completa:
Titolo del libro:
XXV Congresso Nazionale della Società Chimica Italiana : atti del congresso