Validation of ELISA-based detection of L. monocytogenes and E. coli O157:H7 in fresh cut vegetables
Articolo
Data di Pubblicazione:
2014
Citazione:
Validation of ELISA-based detection of L. monocytogenes and E. coli O157:H7 in fresh cut vegetables / M. Cavaiuolo, A. Ferrante, S. Paramithiotis, A. Hadjilouka, P. Tzamalis, E.H. Drosinos. - In: JOURNAL OF FOOD, AGRICULTURE & ENVIRONMENT. - ISSN 1459-0255. - 12:3/4(2014 Oct), pp. 98-99.
Abstract:
Innovative diagnostic methods were developed for the detection and quantification of Listeria monocytogenes and Escherichia coli O157:H7 in
minimally processed fresh cut fruits and vegetables. The aim of the present study was to validate the technical efficiency of these methods and
evaluate their efficacy and viability for routine analysis. To this purpose, ready-to-eat fresh fruits and vegetables were collected throughout the
production chain. A multidisciplinary approach, including a newly developed ELISA method compared to ISO procedures, was applied to detect the
pathogenic bacteria after harvesting, processing and shelf-life. Results obtained exhibited the technical efficiency of the developed methods showing
similar sensitivity, specificity, negative predictive values and negative likelihood ratios.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
ELISA; ISO; Leafy vegetables; Melon; Vegetables
Elenco autori:
M. Cavaiuolo, A. Ferrante, S. Paramithiotis, A. Hadjilouka, P. Tzamalis, E.H. Drosinos
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