Telomere dysfunction in peripheral blood mononuclear cells from patients with primary biliary cirrhosis
Articolo
Data di Pubblicazione:
2014
Citazione:
Telomere dysfunction in peripheral blood mononuclear cells from patients with primary biliary cirrhosis / P. Invernizzi, F. Bernuzzi, A. Lleo, V. Pozzoli, M. Bignotto, P. Zermiani, A. Crosignani, P.M. Battezzati, M. Zuin, M. Podda, C. Raggi. - In: DIGESTIVE AND LIVER DISEASE. - ISSN 1590-8658. - 46:4(2014), pp. 363-368. [10.1016/j.dld.2013.11.008]
Abstract:
BACKGROUND:
Chromosomal instability in peripheral blood mononuclear cells has a role in the onset of primary biliary cirrhosis. We hypothesized that patients with primary biliary cirrhosis may harbour telomere dysfunction, with consequent chromosomal instability and cellular senescence.
AIM:
To evaluate the clinical significance of telomerase activity and telomere length in peripheral blood mononuclear cells from patients with primary biliary cirrhosis.
STUDY DESIGN:
In this population-based case control study, 48 women with primary biliary cirrhosis (25 with cirrhosis), 12 with chronic hepatitis C matched by age and severity of disease, and 55 age-matched healthy women were identified. Mononuclear cells from the peripheral blood of patients and controls were isolated. Telomere length and telomerase activity were measured.
RESULTS:
Telomere length and telomerase activity did not differ between cases (5.9 ± 1.5 kb) and controls (6.2 ± 1.4 kb, pc=0.164). Telomere shortening and advanced-stage disease strongly correlated with telomerase activity. Patients with advanced disease retained significantly less telomerase activity than those with early-stage disease (0.6 ± 0.9 OD vs. 1.5 ± 3.7 OD, p=0.03). Telomere loss correlated with age, suggesting premature cellular ageing in patients with primary biliary cirrhosis.
CONCLUSION:
Our data strongly support the telomere hypothesis of human cirrhosis, indicating that telomere shortening and telomerase activity represent a molecular mechanism in the evolution of human cirrhosis in a selected population of patients
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
autoimmune disease ; primary biliary cirrhosis ; Telomere dysfunction ; rheumatoid-arthritis ; chromosomal instability ; cellular senescence ; immune-system ; diseases ; length ; autoimmunity ; monosomy
Elenco autori:
P. Invernizzi, F. Bernuzzi, A. Lleo, V.M. Pozzoli, M. Bignotto, P. Zermiani, A. Crosignani, P.M. Battezzati, M. Zuin, M. Podda, C. Raggi
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