Hyperacidification of Vacuoles by the Combined Action of Two Different P-ATPases in the Tonoplast Determines Flower Color
Articolo
Data di Pubblicazione:
2014
Citazione:
Hyperacidification of Vacuoles by the Combined Action of Two Different P-ATPases in the Tonoplast Determines Flower Color / M. Faraco, C. Spelt, M. Bliek, W. Verweij, A. Hoshino, L. Espen, B. Prinsi, R. Jaarsma, E. Tarhan, A.H. de Boer, G. Di Sansebastiano, R. Koes, F.M. Quattrocchio. - In: CELL REPORTS. - ISSN 2211-1247. - 6:1(2014 Jan 16), pp. 32-43. [10.1016/j.celrep.2013.12.009]
Abstract:
The acidification of endomembrane compartments is
essential for enzyme activities, sorting, trafficking,
and trans-membrane transport of various compounds.
Vacuoles are mildly acidic inmost plant cells
because of the action of V-ATPase and/or pyrophosphatase
proton pumps but are hyperacidified in
specific cells by mechanisms that remained
unclear. Here, we show that the blue petal color of
petunia ph mutants is due to a failure to hyperacidify
vacuoles. We report that PH1 encodes a P3BATPase,
hitherto known as Mg2+ transporters in bacteria
only, that resides in the vacuolar membrane
(tonoplast). In vivo nuclear magnetic resonance and
genetic data show that PH1 is required and, together
with the tonoplast H+ P3A-ATPase PH5, sufficient to
hyperacidify vacuoles. PH1 has no H+ transport
activity on its own but can physically interact with
PH5 and boost PH5 H+ transport activity. Hence,
the hyperacidification of vacuoles in petals, and
possibly other tissues, relies on a heteromeric
P-ATPase pump.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
P-ATPase pump ; tonoplast ; vacuole ; Petunia ; flower color
Elenco autori:
M. Faraco, C. Spelt, M. Bliek, W. Verweij, A. Hoshino, L. Espen, B. Prinsi, R. Jaarsma, E. Tarhan, A.H. de Boer, G. Di Sansebastiano, R. Koes, F.M. Quattrocchio
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