Regioselective Deacetylation of Disaccharides via Immobilized Aspergillus niger Esterase(s)-catalyzed Hydrolysis in Aqueous and Non-aqueous Media
Articolo
Data di Pubblicazione:
2013
Citazione:
Regioselective Deacetylation of Disaccharides via Immobilized Aspergillus niger Esterase(s)-catalyzed Hydrolysis in Aqueous and Non-aqueous Media / T. Bavaro, P. Torres-Salas, N. Antonioli, C.F. Morelli, G. Speranza, M. Terreni. - In: CHEMCATCHEM. - ISSN 1867-3880. - 5:10(2013), pp. 2925-2931.
Abstract:
Purified lipase fractions from crude commercial extract of Aspergillus niger lipase (ANL) were selectively immobilized on hydrophobic
supports. A significant percentage of esterase activity remains in the supernatant, derived from esterase(s) unable to become adsorbed onto the employed matrices. These residual
proteins were covalently immobilized on epoxy-acrylic resin. Immobilized hydrolases were tested in the hydrolysis of acetylated disaccharides in water-cosolvent systems. ANL-Esterase
was able to catalyze regioselective deprotection of acetylated b-O-methyl lactoside in C-2 position and b-O-methyl lactosaminide in C-3’ position. The hydrolyzed products, never reported before, can be considered new building blocks for the
synthesis of oligosaccharides of biological relevance. Furthermore,
preparative hydrolyses were also performed in tert-butanol.
This solvent is compatible with ANL-esterase stability and it appears to be a novel and promising approach because of its green status.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
biocatalysis ; hydrolysis ; oligosaccharides ; regioselectivity ; supported catalysts
Elenco autori:
T. Bavaro, P. Torres-Salas, N. Antonioli, C.F. Morelli, G. Speranza, M. Terreni
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