Glutathione–enriched baker’s yeast: production, bioaccessibility and intestinal transport assays
Articolo
Data di Pubblicazione:
2014
Citazione:
Glutathione–enriched baker’s yeast: production, bioaccessibility and intestinal transport assays / A. Musatti, V. Devesa, M. Calatayud, D. Velez, M. Manzoni, M. Rollini. - 116:2(2014), pp. 304-313.
Abstract:
AIMS: a glutathione (GSH) yeast-based biomass (S. cerevisiae) was used to investigate GSH stability, solubilization during gastrointestinal digestion and GSH intestinal transport.
METHODS AND RESULTS: a post-growing procedure was applied to improve intracellular GSH yeast content. The presence of adenine (ADE) in the biotransformation solution (CYS-GLY-GLU mixture) and alternatively, a glucose shot after 4 h incubation, allowed to obtain cells containing about GSH 1.6-1.7% dcw (dry cell weight) (control 0.5%). Yeast samples were subjected to in vitro gastro-intestinal digestion and absorption assays employing Caco-2 and HT29-MTX cell lines in different proportions (100/0, 70/30 and 50/50). Trials were also performed to verify intestinal cell viability.
CONCLUSIONS: at least 87% of ingested GSH is available in reduced form for intestinal absorption. In vitro GSH transport assays indicated that GSH is poorly absorbed (<20%). Nevertheless, studies in response to oxidative stress induced by H2 O2 demonstrated a protective role of the GSH-enriched biomass towards intestinal cell viability.
SIGNIFICANCE AND IMPACT OF STUDY: an enriched GSH yeast-based biomass has been obtained using a post-growing procedure. Although GSH present in enriched yeasts is poorly absorbed by intestinal cells, this biomass showed an intestinal local protective effect, improving cells viability when a simulated oxidative stress was applied.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Caco-2; Gastrointestinal digestion; Glutathione; HT29-MTX; Intestinal absorption; Yeast
Elenco autori:
A. Musatti, V. Devesa, M. Calatayud, D. Velez, M. Manzoni, M. Rollini
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