Improvement of Intracellular Glutathione content in Baker’s Yeast for Nutraceutical Application
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Data di Pubblicazione:
2012
Citazione:
Improvement of Intracellular Glutathione content in Baker’s Yeast for Nutraceutical Application / M. Rollini, A. Musatti. ((Intervento presentato al 4. convegno European DoE User Meeting tenutosi a Wien nel 2012.
Abstract:
The study was aimed at investigating the best conditions to increase intracellular GSH levels in samples of baker’s yeast (S. cerevisiae), employed either in compressed and dried form. Glucose, GSH precursors amino acids (cysteine, glycine and glutamic acid), as well as other cofactors, were dissolved in a biotransformation solution in which yeast cells were added (5% dcw). Two response surface central composite designs (RSCCD) were performed in sequence: in the first step the influence of amino acid composition (cysteine, glycine, glutamic acid and serine) on GSH accumulation was investigated; once setup their formulation, the influence of other components was studied. GSH accumulation ability of baker’s yeast in compressed form was found higher at the beginning of shelf life, i.e. in the first week, and a maximum of 2.04%dcw was obtained. Performance of yeast in dried form was not found satisfactory, as the maximum GSH level was 1.18%dcw. When cysteine lacks from the reaction solution, yeast cells did not accumulate GSH. With dried yeast, the highest GSH yields occurred when cysteine was set at 3 g/L, glycine and glutamic acid at least at 4 g/L and serine minimized. Employing compressed yeast, the highest GSH yields occurred when cysteine and glutamic acid were set at 2-3 g/L, while glycine and serine higher than 2 g/L. Results allowed to setup an optimal and feasible procedure to obtain GSH-enriched yeast biomass, with up to 3-fold increase respect to initial content.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
M. Rollini, A. Musatti
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