Development of Genus/Species-Specific PCR Analysis for identification of Carnobacterium strains
Articolo
Data di Pubblicazione:
2002
Citazione:
Development of Genus/Species-Specific PCR Analysis for identification of Carnobacterium strains / M. Scarpellini, D. Mora, S. Colombo, L. Franzetti. - In: CURRENT MICROBIOLOGY. - ISSN 0343-8651. - 45:1(2002), pp. 24-29.
Abstract:
Heterofermentative lactic acid bacteria belonging to the genus Carnobacterium are currently divided into seven different species, C. piscicola, C. mobile, C. gallinarum, C. inhibens, C. divergens, C. funditum, and C. alterfunditum. 16S rDNA-targeted PCR assay was carried out for the identification of the genus Carnobacterium. In addition, type strains of all Carnobacterium species were analyzed by 16S–23S rDNA intergenic spacer analysis in comparison with type strains of phylogenetically related lactic acid bacteria. These methods enabled the identification and the discrimination among Carnobacterium species and the other phylogenetically related lactic acid bacteria. Likewise, analogous results were obtained by restriction analysis of amplified 16S rDNA performed with HaeIII and HinfI as
restriction enzymes.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Carnobacterium ; PCR
Elenco autori:
M. Scarpellini, D. Mora, S. Colombo, L. Franzetti
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