Fate of the oxygen atoms in the diol-dehydratase-catalyzed dehydration of meso-butane-2,3-diol
Articolo
Data di Pubblicazione:
2001
Citazione:
Fate of the oxygen atoms in the diol-dehydratase-catalyzed dehydration of meso-butane-2,3-diol / G. Speranza, C. F. Morelli, M. Orlandi, M. Scarpellini, P. Manitto. - In: HELVETICA CHIMICA ACTA. - ISSN 0018-019X. - 84:2(2001), pp. 335-344. [10.1002/1522-2675(20010228)84:2<335::AID-HLCA335>3.0.CO;2-K]
Abstract:
O-18-Substituted propane-1,2-diols and meso-butane-1,2-diols were synthesized and fed to growing cells of Lactobacillus brevis. Propan-1-ol and butan-2-ol, prepared from such diols through diol-dehydratase-catalyzed dehydration followed by intracellular reduction, were analyzed for their O-18-content. For each propane-1,2-diol enantiomer, partial retention or complete loss of the isotope;appeared to be related to the mode of substrate binding. Specific retention of the O-atom linked to the (R)-configured C-atom of meso-butane-1,2-diol indicates that the diol dehydratase handles this substrate like (R)-propane-1,2-diol.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
direct participation; potassium-ion; X-ray; mechanism; meso-2,3-butanediol; glycerol; complex
Elenco autori:
G. Speranza, C. F. Morelli, M. Orlandi, M. Scarpellini, P. Manitto
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