Data di Pubblicazione:
1988
Citazione:
MODULATION OF HMG-COA REDUCTASE-ACTIVITY BY PANTETHEINE PANTETHINE / G. CIGHETTI, M. DELPUPPO, R. PARONI, M.G. KIENLE. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - 963:2(1988 Nov 25), pp. 389-393.
Abstract:
The ability of pantetheine/pantethine to modulate the aptivity of HMG-CoA reductase (EC 1.1.1.3) was
determined in vitro with rat liver microsomes. The decay of the activity was obtained with pantethine in the
10(ex-5)-10(ex-4) M range, whereas stimulation by panlgtheine occurred at 10(ex-3)-10(ex-2) M, as previously reported for GSSG and GSH, respectively. Inhibition of HMG-CoA by pantethine in isolated liver cells was also investigated by measuring the enzyme activity in microsomes isolated from hepatocytes incubated without or
with 1 mM pantethine under conditions previously shown by us to induce inhibition of cholesterol synthesis
from acetate. The enzyme amount was not modified by pantethine, but in cells treated with the disulphide,
the relative amounts of the thiolic active forms of the enzyme, both phosphorylated and dephosphorylated,
were decreased to about half compared to controls.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
(Rat liver microsomes); Cholesterol synthesis; HMG-CoA reductase; Hydroxymethylglutaryl-CoA reductase; Pantetheine/pantethine
Elenco autori:
G. CIGHETTI, M. DELPUPPO, R. PARONI, M.G. KIENLE
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