Acute and chronic changes in K(+)-induced depolarization alter NMDA and nNOS gene expression in cultured cerebellar granule cells
Articolo
Data di Pubblicazione:
1996
Citazione:
Acute and chronic changes in K(+)-induced depolarization alter NMDA and nNOS gene expression in cultured cerebellar granule cells / F. Tascedda, R. Molteni, G. Racagni, M.A. Riva. - In: MOLECULAR BRAIN RESEARCH. - ISSN 0169-328X. - 40:1(1996 Aug), pp. 171-174. [10.1016/0169-328X(96)00111-8]
Abstract:
The influence of low or high (10 or 25 mM) K(+)-induced membrane depolarization on the mRNA levels for NMDA receptor subunits was investigated by RNase protection assay in cultured rat cerebellar granule cells. Cells, maintained for 7 days in K25+, a condition that promotes their survival and maturation, express the highest levels of NR-1 and NR-2A mRNA, whereas NR-2B is maximally expressed in cells grown in K10+. Acute changes in medium K+ concentration had a significant effect on the mRNA levels for NMDA receptor subunits. A concomitant reduction of NR-2A mRNA and induction of NR-2B was observed following a 24-h shift of the culture medium from K25+ to K10+. Under these circumstances NR-2C, not detected in basal conditions, became expressed. Neuronal nitric oxide synthase, an enzyme linked to NMDA receptor activation, was also influenced by growth conditions. Its expression, higher under low excitation (K10+), is induced in the shift from K25+ to K10+ and is markedly decreased in the opposite situation. These data indicate that several factors may influence the expression of NMDA receptor subunits and consequently may modulate the function of this receptor complex and its adaptation to acute and chronic changes in neuronal activity.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Animals; Cerebellum; Potassium; Transcription, Genetic; Rats; RNA, Messenger; Animals, Newborn; Macromolecular Substances; Rats, Sprague-Dawley; Nitric Oxide Synthase; Cells, Cultured; Neurons; Culture Media; Isoenzymes; Membrane Potentials; Receptors, N-Methyl-D-Aspartate
Elenco autori:
F. Tascedda, R. Molteni, G. Racagni, M.A. Riva
Link alla scheda completa:
Link al Full Text: