Genetic and Molecular Mechanisms Underlying the parthenocarpic fruit (pat) Mutation in tomato
Articolo
Data di Pubblicazione:
2024
Citazione:
Genetic and Molecular Mechanisms Underlying the parthenocarpic fruit (pat) Mutation in tomato / M.E. Picarella, F. Ruiu, L. Selleri, S. Presa, C. Mizzotti, S. Masiero, L. Colombo, G.P. Soressi, A. Granell, A. Mazzucato. - In: FRONTIERS IN PLANT SCIENCE. - ISSN 1664-462X. - 15:(2024), pp. 1329949.1-1329949.16. [10.3389/fpls.2024.1329949]
Abstract:
Parthenocarpy allows fruit set independently of fertilization. In parthenocarpic- Q9
prone tomato genotypes, fruit set can be achieved under pollen-limiting
environmental conditions and in sterile mutants. Parthenocarpy is also
regarded as a quality-related trait, when seedlessness is associated with
positive fruit quality aspects. Among the different sources of genetic
parthenocarpy described in tomato, the parthenocarpic fruit (pat) mutation is
of particular interest because of its strong expressivity, high fruit set, and
enhanced fruit quality. The complexity of the pat “syndrome” associates a
strong competence for parthenocarpy with a complex floral phenotype
involving stamen and ovule developmental aberrations. To understand the
genetic basis of the phenotype, we mapped the pat locus within a 0.19-cM
window of Chr3, comprising nine coding loci. A non-tolerated missense
mutation found in the 14th exon of Solyc03g120910, the tomato ortholog of
the Arabidopsis HD-Zip III transcription factor HB15 (SlHB15), cosegregated with
the pat phenotype. The role of SlHB15 in tomato reproductive development was
supported by its expression in developing ovules. The link between pat and
SlHB15 was validated by complementation and knock out experiments by cosuppression and CRISPR/Cas9 approaches. Comparing the phenotypes of pat
and those of Arabidopsis HB15 mutants, we argued that the gene plays similar
functions in species with fleshy and dry fruits, supporting a conserved
mechanism of fruit set regulation in plants.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
fruit set; HD-Zip III transcription factors; ovule development; parthenocarpy; seedlessness
Elenco autori:
M.E. Picarella, F. Ruiu, L. Selleri, S. Presa, C. Mizzotti, S. Masiero, L. Colombo, G.P. Soressi, A. Granell, A. Mazzucato
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