Multiplexing Biosensor for the Detection of Extracellular Vesicles as Biomarkers of Tissue Damage and Recovery after Ischemic Stroke
Articolo
Data di Pubblicazione:
2023
Citazione:
Multiplexing Biosensor for the Detection of Extracellular Vesicles as Biomarkers of Tissue Damage and Recovery after Ischemic Stroke / S. Picciolini, V. Mangolini, F. Rodà, A. Montesano, F. Arnaboldi, P. Liuzzi, A. Mannini, M. Bedoni, A. Gualerzi. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 24:9(2023 Apr 27), pp. 7937.1-7937.18. [10.3390/ijms24097937]
Abstract:
The inflammatory, reparative and regenerative mechanisms activated in ischemic stroke
patients immediately after the event cooperate in the response to injury, in the restoration of functions
and in brain remodeling even weeks after the event and can be sustained by the rehabilitation
treatment. Nonetheless, patients’ response to treatments is difficult to predict because of the lack
of specific measurable markers of recovery, which could be complementary to clinical scales in
the evaluation of patients. Considering that Extracellular Vesicles (EVs) are carriers of multiple
molecules involved in the response to stroke injury, in the present study, we have identified a panel
of EV-associated molecules that (i) confirm the crucial involvement of EVs in the processes that
follow ischemic stroke, (ii) could possibly profile ischemic stroke patients at the beginning of the
rehabilitation program, (iii) could be used in predicting patients’ response to treatment. By means
of a multiplexing Surface Plasmon Resonance imaging biosensor, subacute ischemic stroke patients
were proven to have increased expression of vascular endothelial growth factor receptor 2 (VEGFR2)
and translocator protein (TSPO) on the surface of small EVs in blood. Besides, microglia EVs and
endothelial EVs were shown to be significantly involved in the intercellular communications that
occur more than 10 days after ischemic stroke, thus being potential tools for the profiling of patients
in the subacute phase after ischemic stroke and in the prediction of their recovery.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
extracellular vesicles; Surface Plasmon Resonance imaging; biosensor; stroke; biomarkers
Elenco autori:
S. Picciolini, V. Mangolini, F. Rodà, A. Montesano, F. Arnaboldi, P. Liuzzi, A. Mannini, M. Bedoni, A. Gualerzi
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