Data di Pubblicazione:
2008
Citazione:
Biomimetic poly(amidoamine) hydrogels as synthetic materials for cell culture / E. Jacchetti, E. Emilitri, S. Rodighiero, M. Indrieri, A. Gianfelice, C. Lenardi, A. Podesta', E. Ranucci, P. Ferruti, P. Milani. - In: JOURNAL OF NANOBIOTECHNOLOGY. - ISSN 1477-3155. - 6:(2008), p. 14.14. [10.1186/1477-3155-6-14]
Abstract:
Background: Poly(amidoamine)s (PAAs) are synthetic polymers endowed with many biologically
interesting properties, being highly biocompatible, non toxic and biodegradable. Hydrogels based
on PAAs can be easily modified during the synthesis by the introduction of functional comonomers.
Aim of this work is the development and testing of novel amphoteric nanosized
poly(amidoamine) hydrogel film incorporating 4-aminobutylguanidine (agmatine) moieties to create
RGD-mimicking repeating units for promoting cell adhesion.
Results: A systematic comparative study of the response of an epithelial cell line was performed
on hydrogels with agmatine and on non-functionalized amphoteric poly(amidoamine) hydrogels and
tissue culture plastic substrates. The cell adhesion on the agmatine containing substrates was
comparable to that on plastic substrates and significantly enhanced with respect to the nonfunctionalized
controls. Interestingly, spreading and proliferation on the functionalized supports are
slower than on plastic exhibiting the possibility of an easier control of the cell growth kinetics. In
order to favor the handling of the samples, a procedure for the production of bi-layered constructs
was also developed by means the deposition via spin coating of a thin layer of hydrogel on a pretreated
cover slip.
Conclusion: The obtained results reveal that PAAs hydrogels can be profitably functionalized and,
in general, undergo physical and chemical modifications to meet specific requirements. In particular
the incorporation of agmatine warrants good potential in the field of cell culturing and the
development of supported functionalized hydrogels on cover glass are very promising substrates
for applications in cell screening devices.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
E. Jacchetti, E. Emilitri, S. Rodighiero, M. Indrieri, A. Gianfelice, C. Lenardi, A. Podesta', E. Ranucci, P. Ferruti, P. Milani
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