Data di Pubblicazione:
2023
Citazione:
Regulatory Role of microRNA of Milk Exosomes in Mastitis of Dairy Cows / B. Stefanon, M. Cintio, S. Sgorlon, E. Scarsella, D. Licastro, A.A. Zecconi, M. Colitti. - In: ANIMALS. - ISSN 2076-2615. - 13:5(2023), pp. 821.1-821.13. [10.3390/ani13050821]
Abstract:
The aim of this study was to compare the cargos of miRNA in exosomes isolated from the
milk of healthy (H) cows, cows at risk of mastitis (ARM), and cows with subclinical mastitis (SCM).
Based on the number of somatic cells and the percentage of polymorphonuclear cells, 10 cows were
assigned to group H, 11 to group ARM, and 11 to group SCM. After isolating exosomes in milk by
isoelectric precipitation and ultracentrifugation, the extracted RNA was sequenced to 50 bp long
single reads, and these were mapped against Btau_5.0.1. The resulting 225 miRNAs were uploaded
to the miRNet suite, and target genes for Bos taurus were identified based on the miRTarBase and
miRanda databases. The list of differentially expressed target genes resulting from the comparisons
of the three groups was enriched using the Function Explorer of the Kyoto Encyclopedia of Genes
and Genomes. A total of 38, 18, and 12 miRNAs were differentially expressed (DE, p < 0.05) in the
comparisons of H vs. ARM, ARM vs. SCM, and H vs. SCM, respectively. Only 1 DE miRNA was
shared among the three groups (bta-mir-221), 1 DE miRNA in the H vs. SCM comparison, 9 DE
miRNAs in the ARM vs. SCM comparison, and 21 DE miRNAs in the H vs. ARM comparison. A
comparison of the enriched pathways of target genes from the H, SCM, and ARM samples showed
that 19 pathways were differentially expressed in the three groups, while 56 were expressed in the H
vs. SCM comparison and 57 in the H vs. ARM comparison. Analyzing milk exosome miRNA cargos
can be considered as a promising approach to study the complex molecular machinery set in motion
in response to mastitis in dairy cows.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
milk; exosomes; miRNA; mastitis; cow
Elenco autori:
B. Stefanon, M. Cintio, S. Sgorlon, E. Scarsella, D. Licastro, A.A. Zecconi, M. Colitti
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