The cyclic peptide G4CP2 enables the modulation of galactose metabolism in yeast by interfering with GAL4 transcriptional activity
Articolo
Data di Pubblicazione:
2023
Citazione:
The cyclic peptide G4CP2 enables the modulation of galactose metabolism in yeast by interfering with GAL4 transcriptional activity / S. Rosa, A. Tagliani, C. Bertaso, L. Tadini, C. Visentin, L.J. Gourlay, S. Pricl, L. Feni, S. Pellegrino, P. Pesaresi, S. Masiero. - In: FRONTIERS IN MOLECULAR BIOSCIENCES. - ISSN 2296-889X. - 10:(2023), pp. 1017757.1-1017757.15. [10.3389/fmolb.2023.1017757]
Abstract:
Genetically-encoded combinatorial peptide libraries are convenient tools to identify peptides to be used as therapeutics, antimicrobials and functional synthetic biology modules. Here, we report the identification and characterization of a cyclic peptide, G4CP2, able to interfere with the GAL4 protein, a transcription factor responsible for the activation of galactose catabolism in yeast and widely exploited in molecular biology. G4CP2 has been identified by screening CYCLIC, a Yeast Two-Hybrid-based combinatorial library of cyclic peptides developed in our laboratory. G4CP2 interferes with GAL4-mediated activation of galactose metabolic enzymes both when expressed intracellularly, as a recombinant peptide, and when provided exogenously, as a chemically-synthesized cyclic peptide.
Our results support the application of G4CP2 in microbial biotechnology and, additionally, demonstrate that CYCLIC can be used as a tool for the rapid identification of peptides, virtually without any limitation with respect to the target protein. The possible biotechnological applications of cyclic peptides are also discussed.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Gal4; Cyclic peptide; Combinatorial library; yeast two-hybrid; Protein interference; Galactose metabolism; Drug Discovery;
Elenco autori:
S. Rosa, A. Tagliani, C. Bertaso, L. Tadini, C. Visentin, L.J. Gourlay, S. Pricl, L. Feni, S. Pellegrino, P. Pesaresi, S. Masiero
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