Enzymatic Synthesis of γ-Glutamyl Dipeptides Catalysed by Mutant E. coli γ-Glutamyltransferases
Articolo
Data di Pubblicazione:
2022
Citazione:
Enzymatic Synthesis of γ-Glutamyl Dipeptides Catalysed by Mutant E. coli γ-Glutamyltransferases / M. Rabuffetti, G. Speranza, C. Calvio, C.F. Morelli. - In: EUROPEAN JOURNAL OF ORGANIC CHEMISTRY. - ISSN 1434-193X. - 2022:43(2022 Oct), pp. e202200907.1-e202200907.9. [10.1002/ejoc.202200907]
Abstract:
g-Glutamyltransferases (GGTs) from different sources have been proposed in recent times as biocatalysts for the enzymatic synthesis of naturally occurring g-glutamyl derivatives with flavor-enhancer properties and interesting biological activities. Although the enzymatic approach is considered as a viable alternative to both the troublesome and low-yielding extraction from natural sources and synthesis through peptide chemistry requiring protection/deprotection steps, yields are not completely satisfactory, due to the intervention of GGT-catalysed hydrolysis and autotranspeptidation side-reactions. Here, the design and the use as biocatalyst for preparative purposes of two mutants of E. coli GGT are described. The design of mutants was pursued by docking-guided identification of residues putatively involved in interaction with the acceptor substrate, thus probably representing a first identification of residues constituting the still elusive and poorly characterized acceptor substrate binding site of the enzyme.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Biocatalysis; Mutagenesis; Amino acids; gamma-glutamyltransferases; Taste-active compounds
Elenco autori:
M. Rabuffetti, G. Speranza, C. Calvio, C.F. Morelli
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